Comparative studies on the analysis of glycosylation heterogeneity of sialic acid-containing glycoproteins using capillary electrophoresis

Comparative studies concerning glycoform analysis of sialoglycoproteins by capillary electrophoresis were performed using a few separation modes hithe rto reported. Glycoprotein samples examined in the present study were succe ssfully separated to their respective glycoforms using surface-modified cap illaries commercially available for capillary gas chromatography in the run ning buffer near their isoelectric points. The analysis times were less tha n 50 min and reproducibilities in migration times were excellent (less than 2.0% RSD for both run-to-run and day-to-day analyses). We present a method for the glycoform analysis of alpha(1)-acid glycoprotein in sera by simple pre-treatment as an application. The present technique will become one of the general methods for the evaluation of glycosylation heterogeneity of co mmercially available glycoprotein drugs. (C) 2000 Elsevier Science B.V. All rights reserved.