M. Hardt et al., A comparative study of immunohistochemical methods for detecting abnormal prion protein with monoclonal and polyclonal antibodies, J COMP PATH, 122(1), 2000, pp. 43-53
Citations number
40
Categorie Soggetti
Veterinary Medicine/Animal Health","Medical Research Diagnosis & Treatment
Transmissible spongiform encephalopathies are associated with the accumulat
ion of abnormal prion protein (PrPSc) in the central nervous system which c
an be detected immunohistochemically. Using a monoclonal antibody (L42) to
an epitope on the first alpha-helix of ruminant PrP, we compared previously
reported immunohistochemical antigen unmasking and "visualization" systems
. Tn addition, a variety of polyclonal and monoclonal antibodies to other e
pitopes:on ruminant PrP were assessed. Antigen unmasking by hydrated autocl
aving and proteinase K treatments, and antigen detection with L42 and an av
idin-biotin complex system, enabled intra- and extra-neuronal PrPSc:to be d
emonstrated in scrapie-affected sheep carrying three different PrP alleles,
as well as in cases of bovine spongiform encephalopathy. (C) 2000 Harcourt
Publishers Ltd.