Enhanced DNA repair, immune function and reduced toxicity of C-MED-100 (TM), a novel aqueous extract from Uncaria tomentosa

Female W/Fu rats were gavaged daily with a water-soluble extract (C-MED-100 (TM)) of Uncaria tomentosa supplied commercially by CampaMed at the doses o f 0, 5, 10, 20, 40 and 80 mg/kg for 8 consecutive weeks. Phytohemagglutinin (PHA) stimulated lymphocyte proliferation was significantly increased in s plenocytes of rats treated at the doses of 40 and 80 mg/kg. White blood cel ls (WBC) from the C-MED-100(TM) treatment groups of 40 and 80 mg/kg for 8 w eeks or 160 mg/kg for 4 weeks were significantly elevated compared with con trols (P < 0.05). In a human volunteer study, C-MED-100(TM) was given daily at 5 mg/kg for 6 consecutive weeks to four healthy adult males. No toxicit y was observed and again, WBC were significantly elevated (P < 0.05) after supplement. Repair of DNA single strand breaks (SSB) and double strand brea ks (DSB) 3 h after 12 Gy whole body irradiation of rats were also significa ntly improved in C-MED-100(TM) treated animals (P < 0.05). The LD50 and MTD of a single oral dose of C-MED-100(TM) in the rat were observed to be grea ter than 8 g/kg. Although the rats were treated daily with ii tomentosa ext racts at the doses of 10-80 mg/kg for 8 weeks or 160 mg/kg for 4 weeks, no acute or chronic toxicity signs were observed symptomatically. In addition, no body weight, food consumption, organ weight and kidney, liver, spleen, and heart pathological changes were found to be associated with C-MED-100(T M) treatment. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.