Background and aim of the study: The excellent results with atrioventricula
r valve reconstruction have stimulated surgeons to attempt reconstruction o
f calcified aortic valves using decalcifying techniques, but long-term resu
lts have been disappointing. The aim of this in vitro study was to evaluate
the surface structure of decalcified aortic valve tissue and its potential
for recalcification.
Methods: Aortic leaflets were removed from 26 patients with aortic stenosis
during elective valve replacement and decalcified by meticulous dissection
. Representative specimens were prepared for scanning electron microscopy (
SEM) and calcium content in the heavily calcified part of the leaflet in bo
th macroscopically non-calcified and decalcified tissue was determined by a
tomic absorption spectroscopy (AAS). Additional probes of 'non-calcified' a
nd decalcified tissue were incubated for two and four weeks with medium con
taining a physiological concentration of calcium to determine their potenti
al for recalcification. As a control, 13 specimens from non-calcified valve
s were incubated according to the same protocol.
Results: All calcified specimens contained high calcium levels (142.70 +/-
53.76 mg/g). Surgical dissection reduced tissue calcium content significant
ly (10.04 +/- 13.43 mg/g). Following two weeks' incubation with calcium, th
ese specimens retained significantly higher levels of calcium (2.88 +/- 5.1
7 mg/g) than the 'non-calcified' specimens (19.17 +/- 7.61 versus 13.49 +/-
6.27 mg/g: p <0.05); after four weeks similar calcium levels were reached
(32.00 +/- 10.27 versus 28.35 +/- 9.84 mg/g; p = NS). Non-calcified tissue
showed the lowest calcium uptake (4.75 +/- 4.55 mg/g and 12.29 +/- 9,43 mg/
g at two and four weeks; p <0.05). SEM revealed a loss of endothelial cover
age in the calcified areas; decalcification led to an irregular fibrillar s
urface. Only parts of the macroscopically normal tissue contained endotheli
al cells, whereas the control tissue showed intact cellular coverage,
Conclusion: Aortic valve decalcification can effectively remove calcificati
ons, but leaves a fibrillar structure that tends rapidly to accumulate calc
ium. Even normal-appearing tissue from diseased valves has a higher potenti
al for calcification than normal valvular tissue. These data support the ob
servation of only limited clinical benefits being derived after aortic valv
e decalcification for aortic stenosis.