HIV-1-specific mucosal CD8(+) lymphocyte responses in the cervix of HIV-1-resistant prostitutes in Nairobi

Understanding how individuals with a high degree of HIV exposure avoid pers istent infection is paramount to MV vaccine design. Evidence suggests that mucosal immunity, particularly virus-specific CTL, could be critically impo rtant in protection against sexually acquired HIV infection. Therefore, we have looked for the presence of HIV-specific CD8(+) T cells in cervical mon onuclear cells from a subgroup of highly HIV-exposed but persistently seron egative female sex workers in Nairobi, An enzyme-linked immunospot assay wa s used to measure IFN-gamma release in response to known class I HLA-restri cted CTL epitope peptides using effector cells from the blood and cervix of HIV-1-resistant and -infected sex workers and from lower-risk uninfected c ontrols. Eleven of 16 resistant sex workers had HIV-specific CD8(+) T cells in the cervix, and a similar number had detectable responses in blood. Whe re both blood and cervical responses were detected in the same individual, the specificity of the responses was similar. Neither cervical nor blood re sponses were detected in lower-risk control donors. HIV-specific CD8(+) T c ell frequencies in the cervix of HIV-resistant sex workers were slightly hi gher than in blood, while in HIV-infected donor cervical response frequenci es were markedly lower than blood, so that there was relative enrichment of cervical responses in HIV-resistant compared with HIV-infected donors. HIV -specific CD8(+) T cell responses in the absence of detectable HIV infectio n in the genital mucosa of HIV-1-resistant sex workers may be playing an im portant part in protective immunity against heterosexual HIV-I transmission .