Improvement of evaporative light scattering detection of ceramides using triethylamine and formic acid in non-aqueous reversed phase liquid chromatography

The use of Porous Graphitized Carbon (PGC) stationary phase has enabled to emphasize the efficiency of the couple triethylamine (TEA) and formic acid (HCOOH) as a response enhancer for the Evaporative Light Scattering Detecti on (ELSD) of ceramides in Non-Aqueous Reversed Phase (NARP) Liquid Chromato graphy. 0.1% v/v of TEA and a stoechiometric amount of HCOOH greatly increa se the intensity of the detection response whatever the nature of the eluen t and the mobile phase composition was. This increase was not proportional to the amount of the TEA and HCOOH added as the response remained stable ab ove 0.1 % v/v (7 mM).