The monoamine oxidase B gene GT repeat polymorphism and Parkinson's disease in a Chinese population

Monoamine oxidase B (MAOB) metabolises dopamine and activates neurotoxins k nown to induce parkinsonism in humans and primates. Therefore the MAOB gene (MAOB; Xp15.21-4) is a candidate gene for Parkinson's disease (PD). Longer length dinucleotide repeat sequences in a highly polymorphic GT repeat reg ion of intron 2 of this gene showed an association with PD in an Australian cohort. We repeated this allele-association study in a population of 176 C hinese PD patients (90 men, 86 women) and 203 age-matched controls (99 men, 104 women). Genomic DNA was extracted from venous blood and the polymerase chain reaction was used to amplify the appropriate regions of the MAOB gen e. The length of each (GT) repeat sequence was determined by 5% polyacrylam ide denaturing gel electrophoresis. There was no significant difference in allele frequencies of the (GT) repeat allelic variation between patients an d controls (chi(2) = 2.48; df = 5, P < 0.75). Therefore the longer length G T repeat alleles are not associated with PD in this Chinese population. Pos sible reasons for the discrepancy between Chinese and Australian population s include a different interaction between this genetic factor and environme ntal factors in the two populations and the possibility that the long lengt h GT repeat alleles may represent a marker mutation, genetically linked to another susceptibility allele in whites but not in Chinese. Methodological differences in the ascertainment of cases and controls in this cohort could also explain the observed differences. Further study is required to determ ine whether the longer length GT repeat alleles are true susceptibility all eles in PD.