Effects of clarithromycin on cultured human nasal epithelial cells and fibroblasts

Objective/Methods: Long-term administration of clarithromycin has been repo rted to be effective in the treatment of chronic sinusitis. To investigate the mechanism underlying the anti-inflammatory activity of clarithromycin, the authors evaluated the effect of clarithromycin on the gene expression o f proinflammatory cytokine and the DNA-binding activity of nuclear factor ( NF)-kappa B in cultured human nasal epithelial cells and fibroblasts, Cells were incubated with endotoxin purified from, nontypeable Haemophilus influ enzae or interleukin (IL)-1 beta in the presence of clarithromycin, Results : Northern blot analysis revealed that clarithromycin suppressed IL-I beta gene expression in human nasal epithelial cells stimulated by H influenzae endotoxin (HIE), Intercellular adhesion molecule-1 gene expression in nasal fibroblasts stimulated by IL-1 beta was also suppressed by clarithromycin. Futhermore, electrophoretic mobility shift assay demonstrated that clarith romycin reduced DNA-binding activity of NF-kappa B in both human nasal epit helial cells and fibroblasts stimulated by HIE or IL-1 beta respectively. C onclusion: The present results suggest that clarithromycin may reduce gene expression of proinflammatory cytokines and adhesion molecules from nasal m ucosa at the transcriptional factor level and exert an anti-inflammatory ef fect on nasal mucosa in chronic sinusitis.