TRIPLES: a database of gene function in Saccharomyces cerevisiae

Using a novel multipurpose mini-transposon, we have generated a collection of defined mutant alleles for the analysis of disruption phenotypes, protei n localization, and gene expression in Saccharomyces cerevisiae, To catalog this unique data set, we have developed TRIPLES, a Web-accessible database of TRansposon-Insertion Phenotypes, Localization and Expression in Sacchar omyces, Encompassing over 250 000 data points, TRIPLES provides convenient access to information from nearly 7800 transposon-mutagenized yeast strains ; within TRIPLES, complete data reports of each strain may be viewed in tab le format, or if desired, downloaded as tab-delimited text files. Each repo rt contains external links to corresponding entries within the Saccharomyce s Genome Database and International Nucleic Acid Sequence Data Library (Gen Bank), Unlike other yeast databases, TRIPLES also provides on-line order fo rms linked to each clone report; users may immediately request any desired strain free-of-charge by submitting a completed form. In addition to presen ting a wealth of information for over 2300 open reading frames, TRIPLES con stitutes an important medium for the distribution of useful reagents throug hout the yeast scientific community. Maintained by the Yale Genome Analysis Center, TRIPLES may be accessed at http://ycmi.med.yale.edu/ygac/triples.h tm.