PseudoBase: a database with RNA pseudoknots

PseudoBase is a database containing structural, functional and sequence dat a related to RNA pseudoknots. It can be reached at http://wwwbio,Leiden Uni v.nl/similar to Batenburg/PKB.html,This page will direct the user to a retr ieval page from where a particular pseudoknot can be chosen, or to a submis sion page which enables the user to add pseudoknot information to the datab ase or to an informative page that elaborates on the various aspects of the database. For each pseudoknot, 12 items are stored, e.g. the nucleotides o f the region that contains the pseudoknot, the stem positions of the pseudo knot, the EMBL accession number of the sequence that contains this pseudokn ot and the support that can be given regarding the reliability of the pseud oknot, Access is via a small number of-steps, using 16 different categories . The, development process was done by applying the: evolutionary methodolo gy for software development rather than by applying the methodology of the classical waterfall model or the more modern spiral model.