Inhibitory interaction of c-Myb and GATA-1 via transcriptional co-activator CBP

Citation
T. Takahashi et al., Inhibitory interaction of c-Myb and GATA-1 via transcriptional co-activator CBP, ONCOGENE, 19(1), 2000, pp. 134-140
Citations number
43
Categorie Soggetti
Onconogenesis & Cancer Research
Journal title
ONCOGENE
ISSN journal
09509232 → ACNP
Volume
19
Issue
1
Year of publication
2000
Pages
134 - 140
Database
ISI
SICI code
0950-9232(20000106)19:1<134:IIOCAG>2.0.ZU;2-C
Abstract
Gene targeting experiments have revealed that transcription factors such as c-Myb and GATA-1 play crucial roles during hematopoietic differentiation. c-Myb is necessary in the immature cells of almost every hematopoietic line age and GATA-1 is essential for the development of the ervthroid lineage. I n addition, CREB-binding protein (CBP) acts as a transcriptional adapter fo r various transcription factors, including c-Myc and GATA-1. In this paper, ,ve show that the transcription factors c-Myb and GATA-1 each inhibit the t ranscriptional activity of the other and that any possible bipartite comple xes c-Myb, GATA-1, and CBP could be formed, but the tripartite complex was hardly formed, The exclusive binding of GATA-1 and c-Myb to CBP is probably the molecular basis for the mutual inhibition of their transcriptional act ivity. Our data suggest that cross-talk between these three factors might b e important for hematopoietic differentiation and that CBP functions as a h ey molecule during the process.