20-HYDROXYECDYSONE 2-BETA-D-GLYCOPYRANOSIDE FROM SEEDS OF XEROPHYLLUM-TENAX

Citation
B. Alison et al., 20-HYDROXYECDYSONE 2-BETA-D-GLYCOPYRANOSIDE FROM SEEDS OF XEROPHYLLUM-TENAX, Biochemical systematics and ecology, 25(3), 1997, pp. 255-261
Citations number
31
Categorie Soggetti
Ecology,Biology
ISSN journal
03051978
Volume
25
Issue
3
Year of publication
1997
Pages
255 - 261
Database
ISI
SICI code
0305-1978(1997)25:3<255:22FSOX>2.0.ZU;2-9
Abstract
Bioassay/RIA-directed HPLC purification of a MeOH extract of the seeds of Xerophyllum tenax yielded 20-hydroxyecdysone and 20-hydroxyecdyson e 2-beta-D-glucopyranoside. the latter being a novel natural product. The structures were elucidated primarily on the basis of 1D and 2D NMR spectroscopic evidence. The ecdysteroid agonist activity of these iso lates was determined on the B-11 tumorous blood cell line of Drosophil a melanogaster. ED50 values for 20-hydroxyecdysone and 20-hydroxyecdys one 2-beta-D-glucopyranoside were found to be 8.0x10(-9) M and 1.3x10( -4) M, respectively. The significance of the presence of ecdysteroid c onjugates in plants is discussed. (C) 1997 Elsevier Science Ltd.