Two different pathways for protochlorophyllide a (Pchlide) reduction in pho
tosynthetic organisms have been proved: one is strictly light-dependent whe
reas the second is light-independent. Both pathways occur in all photosynth
etic cells except in angiosperms which form chlorophyll only through the li
ght-dependent pathway. Most cells belonging to Eubacteria (i.e., the anoxyg
enic photosynthetic bacteria) synthesize bacteriochlorophyll through the li
ght-independent pathway. This review deals with the physiological, biochemi
cal, and molecular biological features of molecules involved in both pathwa
ys of Pchlide reduction.