Mr. Albiach-marti et al., Aphid transmission alters the genomic and defective RNA populations of Citrus tristeza virus isolates, PHYTOPATHOL, 90(2), 2000, pp. 134-138
A total of 14 Spanish isolates of Citrus tristeza virus (CTV) and 1 isolate
from Japan were transmitted by Aphis gossypii, and the subisolates obtaine
d were compared with the source isolates for symptom expression and double-
stranded RNA (dsRNA) pattern. Of the 14 Spanish isolates, 9 showed altered
dsRNA patterns after aphid transmission but only minor variations in the in
tensity of symptoms induced on Mexican lime. Northern blot hybridization wi
th complementary DNA (cDNA) probes corresponding to both the 5' and the 3'
termini of the CTV genomic RNA (gRNA) showed that the dsRNA bands that coul
d be used to discriminate between the dsRNA pattern of the source and the a
phid-transmitted isolates were the replicative forms of defective RNAs (D-R
NAs). Conversely, the Japanese isolate and two subisolates obtained from it
by aphid transmission had the same dsRNA pattern, but one of the subisolat
es induced milder symptoms in several hosts. Dot-blot hybridization with cD
NA probes representing several regions of the gRNA showed that most of the
aphid-transmitted isolates differed from the corresponding source isolate b
y their hybridization pattern. Our results indicate that aphid transmission
often sorts the populations of gRNA variants and D-RNAs present in CTV iso
lates.