B. Kohler et K. Raschke, The delivery of salts to the xylem. Three types of anion conductance in the plasmalemma of the xylem parenchyma of roots of barley, PLANT PHYSL, 122(1), 2000, pp. 243-254
To explore possible pathways for anions to enter the xylem in the root duri
ng the transport of salts to the shoot, we used the patch-clamp method on p
rotoplasts prepared from the xylem parenchyma of barley (Hordeum vulgare L.
) plants. K+ currents were suppressed by tetraethylammonium or N-methylgluc
amine in the solutions in the pipette and the bath, and the permeating anio
ns were Cl- or NO3-. We recorded the activities of three distinct anion con
ductances: (a) an inwardly rectifying anion channel (X-IRAC), characterized
by activation at hyperpolarization and open times of up to several seconds
; (b) a quickly activating anion conductance (X-QUAC), important for anion
efflux at voltages between -50 mV and the equilibrium potential of the prev
ailing anion; and (c) a slowly activating anion conductance (X-SLAC), activ
ating above -100 mV. Both X-IRAC and X-QUAC were permeable for Cl- and NO3-
; X-QUAC was also permeable for malate. The occurrence of X-IRAC became mor
e frequent with an increase in cytoplasmic Ca2+, while the occurrence of X-
QUAC decreased. Anion currents through X-SLAC, and particularly through X-Q
UAC, were estimated to be large enough to account for reported rates of xyl
em loading, which is in accordance with the notion that xylem loading is a
passive process.