Lactate dehydrogenase isoenzyme distribution and patterns in chicken organs

Unlike most mammals, chicken lactate dehydrogenase isoenzymes cannot be sep arated using the 'Titan-Gel' electrophoresis. However, using isoelectric fo cusing at a pH range of 3.0 to 9.0, a good and clear separation of all five isoenzymes was achieved. Generally, three characteristic groups were seen: (a) those having a cathodic domination (breast muscle and serum) with main ly lactate dehydrogenase-5 (b) those having an anodic domination (heart, mu scle, liver, pancreas, kidney, erythrocytes) of mainly lactate dehydrogenas e - 1 and 2 and (c) those with a more uniform distribution (spleen, lung, a nd brain). The total lactate dehydrogenase activity was the highest in the breast muscle, followed by the heart muscle, Liver and serum with the lowes t activities in the lung and pancreas. (C) 1999 Harcourt Publishers Limited .