Rapid detection of glycoprotein G gene for the diagnosis and typing of herpes simplex virus infection in genital herpes

Objective: To develop a new, rapid, and convenient technique for the diagno sis and typing of herpes simplex virus (HSV) in genital herpes (GH) Methods: Using samples from skin vesicle fluid and urogenital mucosal swabs of subjects with GH, conventional polymerase chain reaction (PCR) (directe d to polymerase gene: PCRpG) were compared with a newly developed PCR (dire cted to HSV glycoprotein gene: PCRgG). Both PCR methods were compared with virus isolation culture (VI) with indirect immunofluorescent staining (IIF) . Results: 80 samples from 40 GH patients (25 males) were tested. Positive re sults were seen in 52.5% (42/80) using PCRgG compared with 40% (32/80) by V I. Most of PCRgG positive samples (95.1%) were caused by HSV-2 infection. I n samples from healing lesions, HSV was detected more often by PCRgG, than by VI. The results of typing by PCRgG and IIF:were highly consistent. Concl usion: PCRgG is more sensitive than VE and PCRpG in detecting HSV in urogen ital samples from subjects with GH. PCRgG is a convenient technique for the rapid detection and typing of GH.