STUDY ON MICROEMULSION ELECTROKINETIC CHR OMATOGRAPHY

Microemulsion Electrokinetic Chromatography(MEEKC) is a relatively new capillary electrophoresis technique using a microemulsion as a separa tion carrier. Two kinds of mixture samples were separated by MEEKC and compared with Micellar Electrokinetic Chromatography (MEKC). The effe cts of microemulsion composition on MEEKC separation were studied. The different core phase showed different migration time, efficiency and time window. The separation was also affacted by core phase and cosurf actant concentration. Using microemulsion consisting of 80mmol/L octan e-120mmol/L sodium dodecyl sulphate-900mmol/L 1-butanol-10mmol/L borat e, relative standard deviation (RSD) of migration time was less than 0 .8% and RSD of peak area was less than 3% (n = 6). High separation eff iciency can be obtained if the microemulsion composition is properly c hosen.