Fetal membrane distention - II. Differentially expressed genes regulated by acute distention in vitro

Citation
E. Nemeth et al., Fetal membrane distention - II. Differentially expressed genes regulated by acute distention in vitro, AM J OBST G, 182(1), 2000, pp. 60-67
Citations number
27
Categorie Soggetti
Reproductive Medicine","da verificare
Journal title
AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY
ISSN journal
00029378 → ACNP
Volume
182
Issue
1
Year of publication
2000
Part
1
Pages
60 - 67
Database
ISI
SICI code
0002-9378(200001)182:1<60:FMD-ID>2.0.ZU;2-R
Abstract
OBJECTIVE: This study was undertaken to identify genes with expression up-r egulated by acute distention in the human fetal membranes. STUDY DESIGN: Fetal membrane explants were distended reproducibly in a nove l device in vitro for 4 hours, and suppression subtractive hybridization wa s used to identify the candidate genes for up-regulation of expression in r esponse to this stimulus. The up-regulation in response to distention was c onfirmed by quantitative Northern blot analysis both after a 4-hour in vitr o distention and after labor in vivo. RESULTS: Suppression subtractive hybridization identified 3 genes with expr ession up-regulated by acute distention: an interferon-stimulated gene enco ding a 54-kd protein, the gene for huntingtin-interacting protein 2 (a ubiq uitin-conjugating enzyme), and a novel transcript. Expression of each of th e distention-responsive genes found to be up-regulated in vitro was also up -regulated in fetal membranes in association with labor. CONCLUSIONS: Suppression subtractive hybridization was successfully applied to a complex tissue, the human fetal membranes, and 3 novel distention-res ponsive genes were identified. Both acute in vitro distention and labor in vivo up-regulate expression of at least 3 genes in the human fetal membrane s.