A comparison of vancomycin and sulfated beta-cyclodextrin as chiral selectors for enantiomeric separations of selenoamino acids using capillary electrophoresis with UV absorbance detection

The enantiomeric separation of three selenoamino acids, D,L-selenomethionin e, D,L-selenoethionine and D,L-selenocystine is described. Both sulfated be ta-cyclodextrin and vancomycin have been successfully used to separate all enantiomers of the compounds with UV detection. Reproducible separations, i n terms of peak area and migration time were obtained using sulfated beta-c yclodextrin with reversed polarity and UV detection. With vancomycin as a c hiral selector, reversed polarity was found to be more reproducible than po sitive polarity in terms of peak migration times.