High-performance liquid chromatography and inductively coupled plasma massspectrometry (HPLC-ICP-MS) for the analysis of xenobiotic metabolites in rat urine: application to the metabolites of 4-bromoaniline

The use of HPLC-ICP-MS for the profiling and quantification of the metaboli tes of 4-bromoaniline following reversed-phase gradient chromatography is d emonstrated. In the 0-8 h post dose sample, which contained the highest con centrations of compound-related material, it was possible to detect at leas t 16 metabolites of the compound. The methodology described offers the poss ibility of obtaining metabolite profiles and quantification for drugs and o ther xenobiotics in biological fluids and excreta without the requirement f or radiolabelled tracers.