Determination of thiamine and riboflavin in pet foods and animal feedingstuffs

Authors
Craig, ID Ashby, CW Brown, I Crosby, NT Guffogg, S Henderson, K Hind, C James, K Jenkinson, D Jones, A Joyce, N Langridge, EW Merson, GH Moulder, AA Nuttall, RT Patel, I Revett, S Wagland, AA Wilson, JJ
Citation
Id. Craig et al., Determination of thiamine and riboflavin in pet foods and animal feedingstuffs, ANALYST, 125(2), 2000, pp. 353-360
Citations number
8
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
ANALYST
ISSN journal
00032654 → ACNP
Volume
125
Issue
2
Year of publication
2000
Pages
353 - 360
Database
ISI
SICI code
0003-2654(2000)125:2<353:DOTARI>2.0.ZU;2-K
Abstract
A method is given for the determination of thiamine (vitamin B-1) and ribof lavin (vitamin B-2) in pet foods and animal feedingstuffs. The vitamins are extracted from the food matrix by digestion with 0.1 M hydrochloric acid f ollowed by enzyme digestion with clara-diastase. The filtered solution is a nalysed by reversed-phase HPLC for riboflavin (directly) and thiamine (afte r thiochrome formation) using fluorescence detection.