A positive selection vector combining tetracycline resistance that eliminates the need for bacterial plating

Authors
Matin, MM Hornby, DP
Citation
Mm. Matin et Dp. Hornby, A positive selection vector combining tetracycline resistance that eliminates the need for bacterial plating, ANALYT BIOC, 278(1), 2000, pp. 46-51
Citations number
19
Categorie Soggetti
Biochemistry & Biophysics
Journal title
ANALYTICAL BIOCHEMISTRY
ISSN journal
00032697 → ACNP
Volume
278
Issue
1
Year of publication
2000
Pages
46 - 51
Database
ISI
SICI code
0003-2697(20000201)278:1<46:APSVCT>2.0.ZU;2-2
Abstract
We describe here the construction of a plasmid that combines positive selec tion with tetracycline resistance. The vector comprises a modified version of the gene;:encoding the cytosine-specific DNA methyltransferase MspI and a modified form of the pBR322 tetA(C) gene. The combination of these two ge nes facilitates the selection of recombinant plasmids in broth cultures, th ereby eliminating the need for bacterial plating. (C) 2000 Academic Press.