Indirect atomic absorption spectrometric determination of flavone in bee chrysalis with flow injection continuous-precipitation technique

A continuous-precipitation flow injection system coupled on-line with atomi c absorption spectrometry for the determination of flavone in bee chrysalis has been developed. A complex precipitate was formed by injecting alkaline lead acetate into bee chrysalis samples and was retained on a Perspex filt er followed by elution with nitric acid solution. The extraction method for flavone by the combination of a cold-methanol and alkaline solution was st udied. The chemical conditions and the Fl parameters were studied in detail . The calibration curve was linear over the range 1 - 100 mu g ml(-1). The detection limit was 0.6 mu g ml(-1). The relative standard deviation was 2. 8% (n=9, 10 mu g ml(-1)) and the recovery was 95.8 - 104%.