Estimations of the efficacy and reliability of paternity assignments from DNA microsatellite analysis of multiple-sire matings

It is important for bovine DNA testing laboratories to provide the cattle i ndustry with accurate estimates of the efficacy and reliability of DNA test s offered so that end users of this technology can adequately assess the co st-benefits of testing. To address these issues for bovine paternity testin g, paternity exclusion probability estimates were obtained from breed panel data and were predictive of the efficacy of the DNA tests used in 39 multi ple-sire mating groups, involving 5960 calves and 505 bulls. Paternity test ing of these mating groups has demonstrated that the majority involve a var iable proportion of unknown sires and this impacts on the reliability of si re allocation. Mathematical models based on binomial or beta-binomial proba bility distributions were used to estimate the reliability of single-sire a llocations from multiple-sire matings involving unknown sires. Reliability of 98-99% is achieved when the exclusion probability is 0.99 or greater, af ter allowing for up to 20% unknown sires. When the exclusion probability dr ops below 0.90 and there are 20% unknown sires, the reliability is poor, br inging into question the benefits of testing. This highlights the need for DNA testing laboratories to offer paternity tests with an exclusion power o f at least 99%.