Expression of stress proteins HSP 72 & HSP 32 in response to endotoxemia

Citation
Jp. Ofenstein et al., Expression of stress proteins HSP 72 & HSP 32 in response to endotoxemia, ANN CLIN L, 30(1), 2000, pp. 92-98
Citations number
39
Categorie Soggetti
Research/Laboratory Medicine & Medical Tecnology","Medical Research Diagnosis & Treatment
Journal title
ANNALS OF CLINICAL AND LABORATORY SCIENCE
ISSN journal
00917370 → ACNP
Volume
30
Issue
1
Year of publication
2000
Pages
92 - 98
Database
ISI
SICI code
0091-7370(200001)30:1<92:EOSPH7>2.0.ZU;2-2
Abstract
Pretreatment with heat decreases mortality and acute lung injury in the rat septic shock model, presumably by the production of heat shock proteins (H SP). However, endotoxin, a severe cell stresser, has not been shown to indu ce HSP 70. We investigated the effects of severe endotoxemia on the express ion of specific protective stress proteins, including HSP 72 (inducible HSP 70), HSP 32 (heme oxygenase-1), and HSP 90. Fifteen rats received intraven ously either 3 mg/kg of endotoxin (E. coli O 127:B8 lipopolysaccharide, LPS ) (n=9) or saline (n=6). Two hr later the spleen was removed and splenocyte s were separated into three groups and analyzed for specific HSP by Western blot. In Group 1, both endotoxin-treated and saline-treated splenocytes we re incubated for 3 hr at 37 degrees C. In Group 2, the splenocytes were was hed twice, then heat shocked for 30 min at 42 degrees C and subsequently in cubated for 2.5 hr at 37 degrees C. Tn Group 3, splenocytes were washed twi ce, then incubated for 3.0 hr at 37 degrees C. HSP 30 & HSP 70c (constituti ve) were present in all groups. Consistent with observations by others, HSP 72 was not induced in Group 1. HSP 72 was induced in both the saline-treat ed and endotoxin-treated splenocytes after heating (Group 2). However, in t he absence of heat stress, HSP 72 was present in endotoxin-treated but nor in saline-treated splenocytes after incubation (Croup 3). Conversely, HSP 3 2, while present in Group 1 splenocytes, was not detected in the endotoxin- treated splenocytes of Group 2 and Group 3, but was present in the saline-t reated cells. In conclusion, endotoxemic shock results in induction of HSP 72 and depletion of HSP 32, but only after the cells have been washed and f urther incubated.