Synthesis, antibacterial, antifungal and anti-HIV evaluation of Schiff andMannich bases of isatin and its derivatives with triazole

Isatin (indole 2,3-dione) and its 5-chloro and 5-bromo derivatives have bee n reacted with 3-(4'-pyridyl)-4-amino-5-mercapto-4-(H)-1,2,6-triazole to fo rm Schiff bases and the N-Mannich bases of these compounds were synthesised by reacting them with formaldehyde and several secondary amines. Their che mical structures have been confirmed by means of their IR, H-1-NMR data and by elemental analysis. Investigation of antimicrobial activity of compound s was done by agar dilution method against 27 pathogenic bacteria, 8 pathog enic fungi and anti-HIV activity against replication of HIV-1 (III B) in MT -4 cells. Among the compounds tested 1-(piperidinomethyl) 5-bromo 3-[3'-(4" -pyridyl)-5'-mercapto-4'-(H)-1',2',4'-triazol 4'-yl]imino isatin showed the most favourable antimicrobial activity.