The effect of cysteine mutations on recombinant deacetoxycephalosporin C synthase from S-clavuligerus

Authors
Lee, HJ Lloyd, MD Harlos, K Schofield, CJ
Citation
Hj. Lee et al., The effect of cysteine mutations on recombinant deacetoxycephalosporin C synthase from S-clavuligerus, BIOC BIOP R, 267(1), 2000, pp. 445-448
Citations number
23
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN journal
0006291X → ACNP
Volume
267
Issue
1
Year of publication
2000
Pages
445 - 448
Database
ISI
SICI code
0006-291X(20000107)267:1<445:TEOCMO>2.0.ZU;2-E
Abstract
Cysteines 100, 155, and 197 of recombinant deacetoxycephalosporin C synthas e were mutated to alanine residues. The C100A mutant had properties similar to those of the wild-type enzyme, but mutation of Cys-155 and Cys-197 redu ced enzyme activity with penicillin N and penicillin G to different extents . (C) 2000 Academic Press.