Hj. Lee et al., The effect of cysteine mutations on recombinant deacetoxycephalosporin C synthase from S-clavuligerus, BIOC BIOP R, 267(1), 2000, pp. 445-448
Citations number
23
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Cysteines 100, 155, and 197 of recombinant deacetoxycephalosporin C synthas
e were mutated to alanine residues. The C100A mutant had properties similar
to those of the wild-type enzyme, but mutation of Cys-155 and Cys-197 redu
ced enzyme activity with penicillin N and penicillin G to different extents
. (C) 2000 Academic Press.