Identification of structurally important domains of lipid phosphate phosphatase-1: implications for its sites of action

Lipid phosphate phosphatase-1 (LPP-1) dephosphorylates exogenous lysophosph atidate and thereby regulates the activation of lysophosphatidate receptors and cell division. Mutation of seven amino acids in three conserved domain s of mouse LPP-1 abolished its activity. A glycosylation site was demonstra ted between conserved Domains 1 and 2. LPP-1 is expressed in the plasma mem brane, and the present results demonstrate the active site to be located on the outer surface.