M. Hashii et al., cADP-ribose potentiates cytosolic Ca2+ elevation and Ca2+ entry via L-typevoltage-activated Ca2+ channels in NG108-15 neuronal cells, BIOCHEM J, 345, 2000, pp. 207-215
The effects of cADP-ribose (cADPR), a metabolite of beta-NAD(+), on the ele
vation of cytoplasmic free Ca2+ concentration ([Ca2+](i)) and Ca2+ flux thr
ough voltage-activated Ca2+ channels (VACCs) were studied in NG108-15 neuro
blastoma x glioma hybrid cells. NG108-15 cells were pre-loaded with fura-2
and whole-cell patch-clamped. Application of cADPR through patch pipettes d
id not by itself trigger any [Ca2+](i) rise at the resting membrane potenti
al. A rise in [Ca2+](i) was evoked upon sustained membrane depolarization,
and was significantly larger in cADPR-infused cells than in non-infused cel
ls. This potentiation in the [Ca2+](i) elevation was reproduced by infusion
of beta-NAD(+), and was blocked by s-bromo-cADPR and antagonized by extern
al application of ryanodine or by pretreatment of cells with FK506. Nicotin
amide inhibited beta-NAD(+)-induced, but not cADPR-elicited, potentiation.
[Ca2+](i) increases or Ca2+ influx, measured by Mn2+ quenching, elicited by
the same protocol of depolarization was blocked completely by nifedipine b
ut not by omega-conotoxin. Ca2+ influx in cADPR- or beta-NAD(+)-infused cel
ls was steeper and greater than that in control cells, and was inhibited pa
rtly by ryanodine. In contrast, ryanodine accelerated Ca2+ influx in non-in
fused cells. These results show that cADPR amplifies both depolarization-in
duced [Ca2+](i) increase and Ca2+ influx through L-type VACCs. These result
s suggest that cADPR functions on ryanodine receptors as a direct agonist a
nd also interacts with L-type VACCs as an indirect agonist, i.e. via a retr
ograde signal.