Since its inception, light microscopy has shown the elegance and subtl
ety with which function is expressed in the form of the cells, tissues
, and organs of the plant. Recently, light microscopy has seen a resur
gence in use fueled by advances in microscope design and computer-base
d image analysis. The structural resolution afforded by static, fixed
samples is being increasingly supplemented by approaches using fluores
cent analogs and selective fluorescent indicators, which visualize the
dynamic processes in Living, functioning cells. This review describes
some of these approaches and discusses how they are taking us a step
closer to viewing the intricate complexity with which plants organize
and regulate their functions down to the subcellular level.