M. Rojas et al., Modulation of benzo[a]pyrene diolepoxide-DNA adduct levels in human white blood cells by CYP1A1, GSTM1 and GSTT1 polymorphism, CARCINOGENE, 21(1), 2000, pp. 35-41
The modulation of benzo[a]pyrene diolepoxide (BPDE)DNA adduct levels by pol
ymorphisms in the CYP1A1, GSTM1 and GSTT1 genes was assessed in leukocytes
of Caucasian males. Eighty-nine coke oven workers (35 smokers, 36 ex-smoker
s and 18 non-smokers) were recruited from job categories with different exp
osure levels to polycyclic aromatic hydrocarbons (PAH), together with 44 po
wer plant workers tall smokers) not exposed to PAH, BPDE-DNA adducts were d
etected in 69 of 133 (52%) DNA samples with a 100-fold variation (range 0.2
-44 adducts/10(8) nt) and a median of 1.6 adducts/10(8) nt, All samples wit
h the GSTM1 active genotype (n = 59) and five out of 74 samples with GSTM*0
/*0 (7 %) showed nondetectable adducts (<0.2 adducts/10(8) nt) and 69 of 74
subjects with GSTM1*0/*0 (93%) had detectable adducts (>0.2 adducts/10(8)
nt), The difference in adduct level between the CSTM1*0/*0 and GSTM1 active
genotypes was highly significant (P < 0.0001). No significant difference i
n adduct level between the GSTT1*0/*0 and GSTT1 active genotypes was seen.
All heterozygotes (CYP1A1*1/*2) from subjects of GSTM1 active type did not
have detectable adducts, Among the GSTM1-deficient individuals (n = 69), 42
with the CYP1A1*/*1 genotype showed a lower adduct level (median 1.3, rang
e 0.2-4.1 adducts/10(8) nt) compared with 26 individuals with heterozygous
mutated CYP1A1*/*2 genotypes (median 2.5, range 0.4-6.1 adducts/10(8) nt, P
< 0.015). One individual with low PAH exposure and the rare combination CY
P1A1*2A/*2A-GSTM1*0/*0 showed an extremely high level of 44 adducts/10(8) n
t, Significant differences in detectable adduct levels were found between t
he CYP1A1*1/*1 and CYP1A1*1/*2 genotypes in the exposed group low + medium
(P = 0.01) and for all adduct levels, detectable and non-detectable (set at
a fixed value), in highly exposed individuals and in ex-smokers (P = 0.03)
, whereas no such differences were observed in the control group. Mutated C
YP1A1*1/*2 increased the adduct level in non-smokers from the exposed group
(1.4 versus 2.2 adducts/10(8) nt), but had no effect on the smokers from t
he exposed group (2.3 versus 2.8 adducts/10(8) nt), When all variables were
dichotomized, statistical evaluation showed that CYP1A1 status (P = 0.015)
, PAH exposure (P = 0.003) and smoking (P = 0.005) had significant effects
on adduct levels which increased in the order: CYP1A1*1/*1 < CYP1A1(*1/*2 o
r *2A/*2A); environmental exposure < occupational exposure; non-smokers < s
mokers, whereby adducts increased with cigarette dose and the duration of s
moking. Higher levels of BPDE-DNA adducts in individuals with the combined
CYP1A1(1/*2 or *2A/*2A)-GSTM1*0/*0 genotype suggest that these genotype com
binations are at increased risk for contracting lung cancer when exposed to
PAH.