Monitoring of the synthesis of cis and trans ruthenium macrocyclic complexes by reversed-phase high-performance liquid chromatography (vol 50, pg 105, 1999)

The synthesis of the complex [Ru(cyclam)Cl-2]Cl (cyclam = 1,4,8,11-tetraaza cyclotetradecane) has been monitored by reversed-phase high-performance liq uid chromatography. The analytical results obtained during the reaction hav e shown that it is feasible to identify and isolate the two isomers cis- an d trans- [Ru(cyclam)Cl-2]Cl. The use of an octadecylsiloxy preparative colu mn enabled the separation and purification of these two isomers and the com pounds have been obtained in high purity. The use of reversed-phase high-pe rformance liquid chromatography has afforded complete analytical control of the syntheses of saturated nitrogen-donor macrocyclic complexes of rutheni um, enabling identification of the cis and trans isomers of the complex [Ru (cyclam)Cl-2]Cl.