AN ELECTRON-SPIN-RESONANCE ASSAY OF GLUTATHIONE S-CONJUGATE TRANSPORT

Authors
PULASKI L BARTOSZ G
Citation
L. Pulaski et G. Bartosz, AN ELECTRON-SPIN-RESONANCE ASSAY OF GLUTATHIONE S-CONJUGATE TRANSPORT, Journal of biochemical and biophysical methods, 33(2), 1996, pp. 65-71
Citations number
16
Categorie Soggetti
Biology,Biophysics,"Biochemical Research Methods
Journal title
Journal of biochemical and biophysical methods → ACNP
ISSN journal
0165022X
Volume
33
Issue
2
Year of publication
1996
Pages
65 - 71
Database
ISI
SICI code
0165-022X(1996)33:2<65:AEAOGS>2.0.ZU;2-J
Abstract
A method for studying export of glutathione S-conjugates from cells is proposed based on the use of the spin label tempo-maleimide. This com pound is conjugated intracellularly with glutathione and the concentra tion of the exported conjugate is measured in cell supernatants in an electron spin resonance spectrometer after reoxidation with ferricyani de. This method allows for measurements of micromolar concentrations o f the conjugate and requires low amounts of cells.