C. Scheler et al., Comparison of two-dimensional electrophoresis patterns of heat shock protein Hsp27 species in normal and cardiomyopathic hearts, ELECTROPHOR, 20(18), 1999, pp. 3623-3628
Heat shock protein Hsp27 occurs in a complex pattern in human myocardial ti
ssue. Normal and failing explanted human heart from patients with dilated c
ardiomyopathy (DCM) or ischemic heart failure (IHF), respectively, were ana
lyzed by high resolution two-dimensional electrophoresis (23x30 cm) and Hsp
27 immunostaining. Twelve Hsp27 spots in DCM samples were significantly alt
ered in intensity and ten of these were significantly changed in IHF. Four
spots (h1, h2, h4, h5) in DCM samples and three spots (h2, h4, h5) in IHF a
t a molecular mass of 28 kDa were decreased in intensity. In this study, in
vestigating left ventricles of human myocardium, spot h4 was only detected
in normal heart samples. On the other hand, spots with a lower molecular ma
ss of 27 kDa (h14, h15, h17, h20, h21) and 22-23 kDa (46, h47, h50) increas
ed in intensity in failing hearts, suggesting that some form of Hsp27 degra
dation occurs during heart failure.