Jl. Afonso et Mj. Clifton, Optimization of protein separation by continuous-flow electrophoresis: Influence of the operating conditions and the chamber thickness, ELECTROPHOR, 20(14), 1999, pp. 2801-2809
The separation of protein by continuous-flow electrophoresis is disturbed b
y a number of effects which cause spreading of the protein streams with a l
oss of resolution. Two numerical models have been used to describe the coup
ling between the various spreading phenomena. A simple model takes into acc
ount molecular diffusion, electroosmosis and residence time gradients; the
main model differs from the simple one by taking account of electrohydrodyn
amics. The influence of the separation chamber thickness, the radius of the
sample filament, the field strength, and the residence time is explored. T
he simulations show that each dispersive effect has its own range of predom
inance, so that an optimum is found for the thickness of the separation cha
mber.