Nonaqueous capillary electrophoretic separation of basic enantiomers usingheptakis(2,3-dimethyl-6-sulfato)-beta-cyclodextrin

The enantiomers of 40 basic analytes, mostly pharmaceuticals, were separate d by nonaqueous capillary electrophoresis in acidic methanol background ele ctrolytes using the sodium salt of heptakis(2,3-dimethyl-6-sulfato)-beta-cy clodextrin (HDMS-beta-CD). The effective mobilities, separation selectiviti es, and peak resolution values were determined as a function of the HDMS-be ta-CD concentration in the 0-40 mM range and were found to follow the theor etical predictions of the charged resolving agent migration model (CHARM mo del). Fast, efficient enantiomer separations were achieved for a large numb er of both very hydrophobic and hydrophilic weak bases.