Capillary zone electrophoresis determination of galanthamine in biologicalfluids and pharmaceutical preparatives: Experimental design and artificialneural network optimization

Galanthamine is a third-generation cholinesterase inhibitor used against Al zheimer's disease. New analytical methods for the determination of galantha mine in pharmaceutical preparatives and biological fluids, such as urine an d serum, were developed. An experimental design and artificial neural netwo rk approach were used for method optimization. Thirty-five ppb of galantham ine were determined in serum samples (with addition of 10 mM magnesium chlo ride and using solid-phase preconcentration).