Yj. Kim et Bk. Hwang, ISOLATION OF A BASIC 34-KILODALTON BETA-1,3-GLUCANASE WITH INHIBITORYACTIVITY AGAINST PHYTOPHTHORA-CAPSICI FROM PEPPER STEMS, Physiological and molecular plant pathology, 50(2), 1997, pp. 103-115
A basic beta-1,3-glucanase (34 kDa, pi 9.3) induced in pepper (Capsicu
m annuum L., cv. Hanbyul) stems by mercuric chloride treatment was pur
ified using CM-cellulose cation exchange chromatography, Bio-gel P-60
gel filtration, and FPLC cation exchange and gel filtration chromatogr
aphy. A close serological relationship was found between the pepper 34
kDa beta-1,3-glucanse and tomato beta-1,3-glucanase. The 34 kDa beta-
1,3-glucanase inhibited hyphal growth of the chitin-negative fungus Ph
ytophthora capsici, but did not show antifungal activity against the c
hitin-containing fungi Alternaria mall, Colletotrichum gloeosporioides
, Magnaporthe grisea, and Fusarium oxysporum f.sp. cucumerinum. Treatm
ent with higher amounts of the beta-1,3-glucanase caused lysis of the
hyphal and zoospore cells of P. capsici. Moreover, the 34 kDA beta-1,3
-glucanase acted synergistically with a pepper basic 32 kDa chitinase
in inhibiting hyphal growth off, oxysporum f.sp. cucumerinum and P. ca
psici. (C) 1997 Academic Press Limited.