Smv. Fernandes et al., Spectrophotometric flow injection determination of total phosphorus in beer using on-line UV/thermal induced digestion, FRESEN J AN, 366(1), 2000, pp. 112-115
A flow injection system for the automatic determination of total phosphorus
in beer is described. The developed manifold uses a two-stage photooxidati
on/thermal digestion procedure together with oxidizing and hydrolyzing reag
ents to convert all forms of phosphorus compounds to orthophosphate. Polyph
osphates are hydrolyzed by acid and heat, and organo-phosphates are digeste
d by UV-catalyzed peroxodisulfate oxidation. The orthophosphate formed is t
hen spectrophotometrically determined by the phosphomolybdenum blue reactio
n, using stannous chloride as reducing agent. The results obtained for a se
t of 19 beer samples (with concentrations from 120 to 735 mg P/L) were in g
ood agreement with the reference method, the maximum relative deviation fou
nd being 4.7%. Relative standard deviations for ten consecutive determinati
ons were lower than 1.5%, and a detection limit of 1 mg P/L was achieved.