Combined AFLP and RFLP mapping in two hexaploid oat recombinant inbred populations

A combined RFLP and AFLP map was constructed for hexaploid oat (Avena spp.) . The segregation of AFLP markers was scored in two hexaploid oat recombina nt inbred line (RIL) populations, the 'Kanota' x 'Ogle' RFLP population, an d a population derived from 'Clintland64' and 'IL86-5698', barley yellow dw arf virus (BYDV)-sensitive and BYDV-tolerant lines, respectively. More than 300 AFLP markers were scored in each population, of which 97 could be scor ed in both populations. AFLP markers were linked to RFLP markers in 32 of 3 6 'Kanota' x 'Ogle' RFLP linkage groups. The addition of the AFLP markers t o the 'Kanota' x 'Ogle' RFLP data set combined markers from four pairs of l inkage groups and increased the size of the map from 1402 cM to 2351 cM. Th irty linkage groups were observed in the 'Clintland64' + 'IL86-5698' popula tion, two of which could be consolidated by comparing the maps from both po pulations. The AFLP and RFLP markers showed very similar distributions in t he 'Kanota' x 'Ogle' population with a tendency of each type of marker to c luster with markers of the same type. The placement of a set of AFLP marker s on the 'Kanota' x 'Ogle' linkage map will enrich the RFLP map and allow o thers to relate AFLP markers for agronomically important genes to the refer ence 'Kanota' x 'Ogle' linkage map.