A CAPS marker to assist selection of tomato spotted wilt virus (TSWV) resistance in pepper

The hypersensitive resistance to tomato spotted wilt virus (TSWV) in pepper is determined by a single dominant gene (resistant allele: Tsw) in several Capsicum chinense genotypes. In order to facilitate the selection for this resistance, four RAPD (among 250 10-mer primers tested) were found linked to the Tsw locus using the bulked segregant analysis and 153 F-2 individual s. A close RAPD marker was converted into a codominant cleaved amplified po lymorphic sequence (CAPS) using specific PCR primers and restriction enzyme s. This CAPS marker is tightly linked to Tsw (0.9 +/- 0.6 cM) and is helpfu l for marker-assisted selection in a wide range of genetic intercrosses.