In vitro effects of low-level, low-frequency electromagnetic fields on DNAdamage in human leucocytes by comet assay

The sources for the effects of electromagnetic fields (EMFs) have been trac ed to time-varying as well as steady electric and magnetic fields, both at low and high to ultra high frequencies. Of these, the effects of low-freque ncy (50 / 60 HZ) magnetic fields, directly related to time-varying currents , are of particular interest as exposure to some fields may be commonly exp erienced. In the present study, investigations have been carried out at low -level (mT) and low-frequency (50 Hz) electromagnetic fields in healthy hum an volunteers. Their peripheral blood samples were exposed to 5 doses of. e lectromagnetic Fields (2,3,5,7 and 10mT at 50 Hz) and analysed by comet ass ay. The results were compared to those obtained from unexposed samples from the same subjects. 50 cells per treatment per individual were scored fur c omet-tail length which is an estimate of DNA damage. Data from observations among males were pooled for each flux density for analysis. Al each flux d ensity, with one exception, there was a significant increase in the DNA dam age from the control value. When compared with a similar study on females c arried out by us earlier, the DNA damage level was significantly higher in the li males as compared to the males for each flux density.