A CHROMOGENIC SUBSTRATE FOR THE CONTINUOUS ASSAY OF MAMMALIAN PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE-C

Authors
RUKAVISHNIKOV AV RYAN M GRIFFITH OH KEANA JFW
Citation
Av. Rukavishnikov et al., A CHROMOGENIC SUBSTRATE FOR THE CONTINUOUS ASSAY OF MAMMALIAN PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE-C, Bioorganic & medicinal chemistry letters, 7(10), 1997, pp. 1239-1242
Citations number
17
Categorie Soggetti
Chemistry Inorganic & Nuclear","Chemistry Medicinal
Journal title
Bioorganic & medicinal chemistry letters → ACNP
ISSN journal
0960894X
Volume
7
Issue
10
Year of publication
1997
Pages
1239 - 1242
Database
ISI
SICI code
0960-894X(1997)7:10<1239:ACSFTC>2.0.ZU;2-9
Abstract
The synthesis of a chromogenic substrate for mammalian PI-PLC, D,L-myo -inositol 4,5-bis(dihydrogen phosphate) 1-(4-nitrophenyl hydrogen phos phate) tetrasodium salt (13), is described. Phosphoinositol bisphospha te 13 (NPIP-P-2) is shown to be a substrate for human PI-PLC delta(1) in a nonradioactive, continuous assay. (C) 1997 Elsevier Science Ltd.