Quantitative IgE inhibition experiments with purified recombinant allergens indicate pollen-derived allergens as the sensitizing agents responsible for many forms of plant food allergy

Citation
L. Kazemi-shirazi et al., Quantitative IgE inhibition experiments with purified recombinant allergens indicate pollen-derived allergens as the sensitizing agents responsible for many forms of plant food allergy, J ALLERG CL, 105(1), 2000, pp. 116-125
Citations number
42
Categorie Soggetti
Clinical Immunolgy & Infectious Disease",Immunology
Journal title
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
ISSN journal
00916749 → ACNP
Volume
105
Issue
1
Year of publication
2000
Part
1
Pages
116 - 125
Database
ISI
SICI code
0091-6749(200001)105:1<116:QIIEWP>2.0.ZU;2-6
Abstract
Background: Type I allergic symptoms in the oropharyngeal mucosa upon conta ct with plant-derived food in patients with pollen allergies have been term ed oral allergy syndrome (OAS). IgE cross-reactivity between pollen and foo d allergens represents the molecular basis for this phenomenon. The sensiti zing allergen source (pollen or plant Toed) in OAS is a controversial issue . Objective:We sought to determine the primary sensitizing molecules in patie nts with GAS. Methods: We used recombinant birch pollen (rBet v 1 and rBet v 2) and plant food allergens (apple, rMal d 1; celery, rApi g 1; and carrot, rDau c 1), as well as natural pollen (birch and timothy grass) and plant food (apple, peach, kiwi, hazelnut, celery, and carrot) allergens, to identify cross-rea ctive allergens by using qualitative immunoblot inhibitions. In addition, w e determined the percentage of plant food-specific IgE that can be preadsor bed with recombinant and natural pollen allergens by quantitative RAST inhi bitions by using sera from 71 patients with GAS. Results: Preincubation of sera with recombinant and natural pollen allergen s led to an almost complete inhibition of IgE binding to plant food allerge ns in Western blots, as well as in RAST inhibition experiments. In contrast , recombinant plant food allergens poorly inhibited IgE binding to Eet v 1. Conclusion: Most IgE epitopes in plant food recognized by patients with OAS are resembled by pollen allergens. Thus pollen allergens may be responsibl e for the elicitation and maintenance of GAS.