Arisugacins C and D, novel acetylcholinesterase inhibitors and their related novel metabolites produced by Penicillium sp FO-4259-11

The mutant of Penicillium sp. FO-4259, an arisugacins A and B producing str ain, was found to produce a series of metabolites, designated arisugacins C , D, E, E G and H, which were structurally related to arisugacins A and B. These compounds were isolated from the culture broth and the physico-chemic al and biological properties were examined. The IC50 values of arisugacins C and D against acetylcholinesterase (AChE) were 2.5 mu M and 3.5 mu M, res pectively. However arisugacins El F, G and H did not inhibit AChE at I OD m u M. Though they showed only weak or no activity against AChE compared with arisugacins A and B, they may be useful for the study of the structure-act ivity relationship.