Reentrant condensation of DNA induced by multivalent counterions

A theory of condensation and resolubilization of a dilute DNA solution with growing concentration of multivalent cations, N, is suggested. It is based on a new theory of screening of a macroion by multivalent cations, which s hows that due to strong cation correlations at the surface of DNA the net c harge of DNA changes sign at some small concentration of cations N-0. DNA c ondensation takes place in the vicinity of N-0, where absolute value of the DNA net charge is small and the correlation induced short range attraction dominates the Coulomb repulsion. At N > N-0 positive DNA should move in th e opposite direction in an electrophoresis experiment. From comparison of o ur theory with experimental values of condensation and resolubilization thr esholds for DNA solution containing Spe(4+), we obtain that N-0 = 3.2 mM an d that the energy of DNA condensation per nucleotide is 0.07 k(B)T. (C) 200 0 American Institute of Physics. [S0021-9606(00)51305-1].