A modular separation-based fiber-optic sensor (SBFOS) with an integrated el
ectronically controlled injection device is described for potential use in
remote environmental monitoring. An SBFOS is a chemical monitor that integr
ates the separation selectivity and versatility afforded by capillary elect
rophoresis with the remote and high sensitivity capabilities of fiber-optic
-based laser-induced fluorescence sensing. The detection module of the SBFO
S accommodates all essential sensing components for dual-optical fiber, on-
capillary fluorescence detection. An injection module, similar to injection
platforms on micro-analysis chips, is also integrated to the SBFOS. The in
jection module allows for electronically controlled injection of the sample
onto the separation capillary. The design and operational characteristics
of the modular SBFOS are discussed in this paper. A micellar electrokinetic
capillary chromatography mode of separation is employed to evaluate the po
tential of the sensor for in situ monitoring of neutral toxins (aflatoxins)
. The analytical figures of merit for the modular SBFOS include analysis ti
mes of between 5 and 10 min, separation efficiencies of approximately 10(4)
theoretical plates, detection limits for aflatoxins in the mid-to-low nano
molar range, and controllable operation that results in sensor performance
that is largely immune to sample matrix effects.