M. Kucukaydin et al., Detection of intestinal bacterial translocation in subclinical ischemia-reperfusion using the polymerase chain reaction technique, J PED SURG, 35(1), 2000, pp. 41-43
Background/Purpose: The purpose of this study was to evaluate the detection
of bacterial translocation after subclinical ischemia reperfusion injuries
in rats with the polymerase chain reaction (PCR) technique.
Methods: Six-week-old weaning rats were divided into 3 groups. (1) Experime
nt rats (n = 20) were gavaged with 10(10) Escherichia coil followed by supe
rior mesentery artery occluded for 10 minutes, then reperfused for 30 minut
es. (2) Control rats (n = 20) received bacterial gavage, (3) Group 3 were s
ham rats (n = 20). After the procedure, 3 mt of blood was obtained from the
portal vein. The terminal ileum and mesenteric lymph node (MLN) near the t
erminal ileum were removed. E coil DNA was detected in blood and MLN sample
s by PCR, and histological changes were examined.
Results: E coli DNA detection in ischemia-reperfusion (VR) group animals wa
s 6 of 20 (30%) in the MLN and 2 of 20 (10%) in the blood. PCR was negative
in all the rats in the control group and in the sham group (P <.05). There
were no significant differences in the histological examination of rat int
estines.
Conclusion: These data suggest that subclinical intestinal I/R injury resul
ts in bacterial translocation, Also, PCR is a highly sensitive and rapid me
thod to detect the presence of microbial DNA. Copyright (C) 2000 by W.B. Sa
unders Company.