We assessed the potential application of near infrared (NIR) spectroph
otometry using a remote optic fiber probe for the development of calib
rations. We evaluated the heat denaturation of whey proteins, the chem
ical composition of a whey protein concentrate (WPC) during production
, and the degree of hydrolysis (DH) of whey proteins during trypsin hy
drolysis. Partial least square (PLS) calibrations were developed for t
hese applications and were related to a variety of products and proces
sing conditions, so as to increase their reliability. NIR has potentia
l applications in real time analysis for whey processing lines.